The timsTOF SCP is the culmination of Bruker’s collaboration with the laboratory of Professor Matthias Mann at the Max Planck Institute of Biochemistry in Martinsried, Germany, and with Evosep on new Whisper methods at ultra-low flow rates of ~100 nL min–1. The timsTOF SCP achieves five times higher ion transmission for data-independent (dia)-PASEF or parallel reaction monitoring (prm)-PASEF methods. The dia-PASEF2 method has demonstrated true, unbiased 4D-proteomics from single cells with quantitation of ~1500 proteins/cell, in hundreds of isolated single cells ex situ, in combination with the Evosep One LC with Whisper. The timsTOF SCP is a dedicated ultra-high sensitivity instrument for unbiased single cell proteomics, after laser capture microdissection of one or a few cells, as well as for ultra-sensitive neoantigen discovery in immunopeptidomics in the field of immunooncology research. With the timsTOF SCP, a system is now available to expand the single-cell biology horizons beyond genomics and transcriptomics to unbiased, quantitative 4D-proteomics. Quantitative biology can now be done also with unbiased single cell proteomics, as cells at different stages in the growth cycle have a protein core with sufficient copy numbers to observe statistically relevant changes in abundance, when compared to sparse mRNA copy numbers.