Bruker’s timsTOF™ Pro has been enhanced by combining PASEF® with parallel reaction monitoring (PRM) for label-free quantitative proteomics. This mode of prm-PASEF takes advantage of the fourth dimension of separation using TIMS to improve selectivity and sensitivity, combined with the speed of PASEF to increase the number of precursor targets. Skyline software can now analyse the prm-PASEF data and produce quantitative reports.
prm-PASEF mode for translational quantitative 4D proteomics
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