The new Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer features advancements that improve system sensitivity over previous generations and expand its ability to characterise and quantify complex biomolecules and biological systems. These enhancements are suitable not only for native omics and translational research, but also for the structural and biopharmaceutical analysis of intact proteins and their complexes. The real-time capabilities of the Orbitrap Eclipse Tribrid use data acquisition strategies to enhance experimental efficiency and ultimately accelerate tandem mass tagging (TMT) for multiplexed quantitative analysis of proteomes. Peptide spectra are searched in real-time against a study-specific database. Those that match then proceed for further analysis, significantly increasing the speed of analysis and accuracy of results. In addition, the new system extends structural analysis up to m/z 8000, which enables the isolation and selective dissociation of protein complexes into their individual components, as well as revealing further insights about their exact structure.