The growing use of cellular and biomolecular processes in energy, medical and environmental applications has created a need for real-time sensing of both reactants and products through process analytical technology (PAT). Raman is already an established process monitoring tool used in PAT, providing excellent analytical selectivity while being particularly insensitive to water present as solvent or moisture within the sample. In this application note, the use of Raman to monitor a simple bioprocess is explored: fermentation of glucose (a common feedstock) with yeast, a microorganism often used in biotechnology. The results show how a sensitive, stable Raman system based on the WP 785 spectrometer can be successfully applied in PAT for bioprocessing applications, even when fluorescence background is present.
Raman process monitoring