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Monitoring cell culture media variability using A-TEEM

Diagram of monitoring cell culture media

Cell culture media for bioreactors provide everything a cell line needs for optimal growth and product yield. It is important to maintain their composition consistent as even subtle variations could have a noticeable impact on the growth rate of the cell culture and its yield. The biopharmaceutical industry has begun to turn to spectroscopic methods, such as fluorescence, for cell culture media characterisation analysis due to the speed of testing, minimal sample handling requirements and relatively lower cost when compared to mass spectrometry and chromatography. In this application note, HORIBA presents A-TEEM (Absorbance Transmission Excitation-Emission Matrix) fluorescence spectroscopy to characterise cell media and the effect that storage conditions have on their compositions.