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How Inner-Filter Effects (IFE) can affect your fluorescence measurements: A case study—colloidal InP quantum dots

Diagram of the process

The applications of quantum dots (QDs) are rapidly expanding, i.e. in vivo imaging, light-emitting devices, photodetection or solar energy conversion, and they require precise knowledge of the QDs emission properties. The Inner Filter Effects (IFEs) can significantly affect fluorescence emission spectral profiles, distorting their general shape, shifting the spectral position of the peak maxima and decreasing the emission intensities. In this application note, the Duetta™ 2-in-1 fluorescence and absorbance spectrometer can measure true molecular fingerprints, which requires the simultaneous acquisition of fluorescence and absorbance, correcting for IFE in real-time.