The CESI 800 high-performance separation-ESI module with OptiMS technology from Beckman Coulter consists of a commercial CESI sprayer combined with new capillary electrophoresis instrumentation specifically designed for mass spectrometry. The new technology integrates a low flow CE separation with ESI in a single process that takes place within a capillary. The new process is referred to as CESI to reflect the combination. To achieve CESI, the distal end of the capillary is porous to ion flow, allowing the electrophoresis circuit to be bridged and the ESI to be initiated in the same device. It is capable of generating separations of peptides with a high peak capacity allowing the analysis of complex samples at the proteome level. The low flow rates used by CE reduce ion suppression, making it possible to detect PTMs. It can also detect peptides present in low abundance at concentrations in the picomolar range. Intact proteins, their isoforms, cleaved fragments and complexes can be characterised and separations of drugs, drug metabolites, organic acids, amino acids, low molecular weight amines, peptides, nucleic acids and nucleosides can also be carried out. Molecules often missed or undetectable can now be seen.

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