Thermo Fisher Scientific has developed a new method for sensitive, accurate and reproducible quantitation of low levels of PFCs in human breast milk. Using selected reaction monitoring and highly-selective reaction monitoring on a UHPLC/MS platform enables reliable and robust detection of PFCs at ppt concentrations. The new method is detailed in an application note entitled Sensitive and Accurate Quantitation of Perfluorinated Compounds in Human Breast Milk using Selected Reaction Monitoring Assays by LC/MS/MS. Using the integrated UHPLC/MS platform, separation of the six PFC analytes in a spiked human breast milk matrix was achieved within nine minutes. As the majority of interferences from matrices elute early at void volume, elution of the first compound at 3.64 minutes ensured a robust quantitation method.
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