Bruker's maXis ETD Sets New Records for Intact Protein Characterisation and Top-down Sequencing

Bruker and several collaborating scientists are presenting a number of milestone achievements in the analysis of intact proteins, including the direct sequencing and characterisation using Bruker's new maXis ETD system. The high mass accuracy, resolving power and dynamic range which are essential to dissect complex patterns of product ions in protein fragmentation allowed, for the first time, performing ETD-based tandem mass spectrometry on biomolecular ions above 60 kDa routinely. These results represent a new record for the mass spectrometric sequencing of intact proteins and unequivocally demonstrate the dramatic advantages of maXis ETD technology in ETD-based top-down protein structural analysis.

"An increasing proportion of new therapies in pharmaceutical development are proteins. At the same time, many first-generation biotherapeutics will soon come off patent protection, and thus the need for rapid and reliable total protein characterization has never been higher," commented Dr Michael Schubert, Executive Vice President at Bruker Daltonics. "We believe that the new maXis ETD has unique and unprecedented capabilities that far exceed what's feasible with intact protein ETD on ion trap/orbitrap hybrid instruments."

Several examples will be demonstrated at Bruker's Users Meeting on Sunday and during the ASMS scientific programme. The recent transfer of Bruker's ETD technology to maXis has allowed precise intact protein analysis as well as comprehensive direct protein gas-phase sequencing. An example of particular note is the direct ETD sequence analysis of intact proteins like serotransferrin and monoclonal antibodies up to a MW of 150 kDa featured by Professor Yury Tsybin of the EPFL (Swiss Federal Institute of Technology Lausanne) in his Thursday 8:30 AM oral presentation at ASMS. Further examples of top-down intact protein identification and characterisation will be presented in various poster sessions at ASMS.
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