“Elucidating structural and compositional changes in plant tissues and single cells by Raman spectroscopic imaging” is the topic of the next article by Batirtze Prats Mateu, Barbara Stefke, Marie-Theres Hauser and Notburga Gierlinger. Understanding plant cells is important for the best use of plants in traditional and new applications. Raman spectroscopic imaging represents one of the best ways to unravel the molecular structure in the native environment of plant tissues.
Hans Lohninger and Johannes Ofner describe “Multisensor hyperspectral imaging as a versatile tool for image-based chemical structure determination”. They describe the features of a software package that allows the combined analysis of hyperspectral data from different imaging techniques. This multisensor approach providing complementary information has many advantages.
by Bill George
University of Glamorgan, UK
How strange the beauty of the vision in the sky.
The bow of colours strong with lurking weaker bow above.
With constancy and symmetry divided by dark space.
And brightness changing with the rain.
A low sun behind us as we watch.
The arc will change from full when sun is low,
and disappear below the earth or sea as day comes on.
With winter rain and sun, it will be found.
In summer rain the bow is lost,
soon after dawn and shortly before dusk.
Symbolism rides high in media and in prose.
We follow Dorothy’s “yellow brick road” to Oz.
“Somewhere over the rainbow” hopes are raised.
But the pot of gold can never to a human be shown
using a moving eye at the apex of a moving cone.
But stranger still the unseen rays beyond the bow.
Beyond the red the rays of radio,
and stronger rays beyond the blue
show inner images which our bodies own,
but damage life when subject to excess.
Who gave us theory for this to track?
Newton, Maxwell, Einstein, Michelson, Dirac?
Some see the ray as wave-like and some a particle.
All agree the speed is fast and constant in emptiness
but slowed when passing through a substance.
The raindrop slows the red more than the blue
and separates each coloured image to the eye.
Absorption gives each object seen its special hue.
Tiny is the fraction of these sights we comprehend
from wider measured but unseen experiment.
Combusted carbon based fossil fuel enters the air.
Crude oil from the earth invades the land and sea.
The population of man increases beyond compare.
Creatures, plants and minerals meet our needs
for food and energy which soon may not be there.
Genesis links the image seen by Noah to a message from
“I do set my bow in the cloud... This is the token of the
between me and you... and the waters shall no longer
become a flood”
Extension of rainbow observation sees a growing amount
of damage, with little time to save the life we understood.
Robert Bradshaw and Simona Francese tell us about “Matrix–assisted laser desorption ionisation tandem mass spectrometry imaging of small molecules from latent fingermarks“. Especially when looking at small molecules in fingermarks, isobaric species can be a problem and this has the potential to affect the outcome of any court case if not handled appropriately. Tandem mass spectrometry can be used as an alternative to high-resolution MS and ion mobility.
Orthogonal spectroscopic techniques for the early developability assessment of therapeutic protein candidates” are described by Patrick Garidel, Anne Karow and Michaela Blech. Due to its cost and time implications, in the early development phase of drug discovery the use of othogonal techniques, based on different physical observables, is important for correct decision-making.
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- Atomic absorption
- Atomic emission
- Ion mobility
- Laser spectroscopy
- Mass spectrometry
- Near infrared
- NMR ESR EPR
- Related equipment
- RMs and standards
- Sample prep
- Separation science
- Surface analysis
- X-ray spectrometry