A fully automated quantitative assay for β-lactam antibiotics in milk has now been developed using on-line SPE-LC-MS/MS. A Spark Holland Symbiosis system for SPE-LC in combination with Applied Biosystems Sciex 3200 triple-quadrupole mass spectrometer for MS/MS enables measurement of target compounds well below the maximum residue limits set by the European Union. Ideal for food control and safety, this newly developed methods offers high sensitivity and accuracy of results, minimum sample pre-treatment and, for the first time, uses an automated on-line SPE offering high throughput analysis.

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